BEGIN:VCALENDAR VERSION:2.0 PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4// BEGIN:VEVENT UID:20250627T170645EDT-8786AUBPoL@132.216.98.100 DTSTAMP:20250627T210645Z DESCRIPTION:Cellular cartography: Mapping protein transport and interaction s in living cells with fluorescence imaging & fluctuation analysis\n\nPaul Wiseman\n\n秀色直播\n\n聽\n\n\n\nImage correlation methods are an extension of fluorescence fluctuation spectroscopy that can measure prote in-protein interactions and macromolecular transport properties from input fluorescence microscopy images of living cells. These approaches are base d on space and time correlation analysis of fluctuations in fluorescence i ntensity within images recorded as a time series using a fluorescence micr oscope. We previously introduced spatio-temporal image correlation spectro scopy (STICS) which measures vectors of protein flux in cells based on the calculation of a spatial correlation function as a function of time from an image time series.\n\nHere we will describe the application of time win dow STICS and its two color extension\, spatio-temporal image cross-correl ation spectroscopy (STICCS)\, for measuring聽cellular waves of adhesion rel ated macromolecules talin and vinculin as well as cytoskeletal actin betwe en assembling and disassembling podosomes in dendritic immune cells. Podos omes are cylindrical membrane complexes with an integrin adhesive ring and an actin rich core that are associated with cellular migration and invasi on in specific cell types. EM and super-resolution microscopy of cells sho ws radial actin filaments that connect neighboring podosomes so we applied pair vector correlation analysis to further characterize the transport wa ves within connected podosome clusters.\n\nThese analyses combined with ph armacological perturbation experiments show that podosome turnover is coor dinated within local clusters in cells with a correlation length scale ext ending to next nearest neighbor podosomes. As well I will introduce future extensions of ICS including new extensions to super-resolution fluorescen ce microscopy.\n\n聽\n DTSTART:20180301T170000Z DTEND:20180301T180000Z LOCATION:908\, McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3 655 promenade Sir William Osler SUMMARY:QLS/CAMBAM Seminar - Dr. Paul Wiseman URL:/qls/channels/event/qlscambam-seminar-dr-paul-wise man-285333 END:VEVENT END:VCALENDAR